When cloning a gene using plasmid vectors, the bacterial colonies containing the recombinant...

Question:

Bacteriophage:

The bacteriophage is also called virus of bacteria. As the virus remains inactive outside the host system, the bacteriophage is also inactive outside the host system and only shows activity when it enters into bacteria. Phage doesn't have any enzymes for transcription, Translation, replication system. Only a phage has its genome and protein coat. Once it infects bacteria, like a virus; it also hijacks the bacterial machinery, recruits bacterial enzymes and protein for its own benefit (to replicate its DNA, transcribe and translate the proteins). Bacteriophage has the gene for its own replication and for synthesis of coat proteins. Phage can stay inside bacteria in 2 forms (i) in lysogenic phase it will go and integrate into the genome and will replicate along with bacterial genome, (ii) in Lytic phase, Phage will infect, synthesize its own macromolecules by hijacking bacterial machinery and lyse the bacteria and comes out. Mostly during cloning lysogenic genes will be deleted from the phage genome.

https://www.ncbi.nlm.nih.gov/books/NBK21450/figure/A1565/?report=objectonly

Answer and Explanation: 1