What are the differences and similarities between cDNA and mRNA? Why is cDNA used as a template in RT-PCR, not mRNA?

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Genomics:-

It is a study of whole genome of organisms and incorporate elements from genetics . They use combination of rDNA and bioinformatics sequence to assemble and analyze structure of function of genomics.

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cDNA stands for Complimentary DNA and mRNA stands for messenger RNA. cDNA is a DNA copy of mRNA molecule which is produced by reverse transcriptase ,...

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DNA Polymerase: Definition & Function

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Chapter 11 / Lesson 5

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DNA polymerase catalyzes the process of DNA replication and repair during cellular respiration. Learn about DNA polymerase function, structure, and types.

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How does mRNA differ from DNA?
What is a difference between DNA replication and RNA transcription?
How are DNA and mRNA alike?
How does RNA differ from DNA in structure?
What is the difference between PCR and DNA sequencing?
Explain how RNA is converted into cDNA, and cDNA is converted into mRNA.
In what way does RNA polymerase differ from DNA polymerase?
How does RNA differ from DNA?
What is the difference between RNA and DNA extraction?
What are three structural differences between DNA and RNA?
Describe one similarity between DNA and RNA.
What is the difference between introns and exons?
What are some similarities between transcription and DNA replication?
Why DNA is transcribed into mRNA to synthesize proteins? Explain.
How does RNA structure differ from DNA structure?
What is the difference between 26S and 28S ribosomal DNA?
What is the difference between DNA replication and transcription?
What is the difference between transcription and DNA replication?
How does the sugar in a DNA nucleotide differ from that of an RNA nucleotide?
What is a major difference between eukaryotic DNA replication and prokaryotic DNA replication?
How are DNA replication and protein synthesis different?
What is the difference between RNA extraction and RNA purification? What is the main difference between DNA extract and RNA extract, and for researches purposes, are they used?
What are the advantages and disadvantages of using RT-PCR and DNA microarray for mRNA gene expression? Which technique is more accurate for indicating the mRNA gene expression?
How are the structures of RNA and DNA similar?
what is the difference between DNA Polymerase 1 and 3?
Is the difference between RNA and DNA that the RNA duplicates itself in the cytoplasm and the DNA duplicates itself in the nucleus? Explain why or why not.
Why is the DNA Sequence of a gene not equivalent to the RNA sequence of the same gene in eukaryotes? What processes generate these differences?
What are the advantages to having DNA, RNA, and protein compared to only RNA?
How are DNA and messenger RNA alike?
How are protein synthesis and DNA replication similar?
How does HIV replication differ from other RNA virus replications?
How does protein electrophoresis differ from DNA electrophoresis?
A) What are DNA and RNA? B) How are they alike and different? C) How do they help each other in the process of replication? Elaborate your answer.
How does Taq polymerase differ from human DNA polymerase?
How does the base composition of DNA and RNA differ?
Why must a mRNA copy be made for protein synthesis?
Explain the process of transcription, particularly the role of mRNA.
How is ribosomal RNA different from other forms of RNA?
What is a major difference between DNA polymerase I and DNA polymerase III?
What is the difference between ligases and DNA polymerase?
How is protein synthesis different from DNA replication?
How is PCR different from DNA replication?
Describe the following: A. DNA B. RNA. C. mRNA.
What are some differences between PCR and in vivo cloning? Is in vivo cloning the same as colony PCR? Provide details.
How does the DNA of prokaryotic cells differ from the DNA of eukaryotic cells?
How are DNA and RNA structurally similar?
In transcription, only one of the DNA strands is "copied" into an mRNA strand. Why not copy both strands so that it would be faster and more efficient?
Explain the role of introns in mRNA regulation.
Explain the process of RNA being translated into proteins. Does tRNA match up with the RNA to make codons?
How does RNA polymerase know which strand of DNA to use as template?
How is DNA structure similar to a protein?
a) What does antisense strand mean? b) Is the template strand same as the antisense strand and non coding strand? c) Are coding strand, sense strand and non template strand the same thing? Explain.
How do you determine which mRNA sequence is the template strand?
How do mRNA and tRNA work together during protein synthesis?
What is the relationship between the template strand and the mRNA transcribed from it?
Explain why DNA synthesis in the lagging strand is different from DNA synthesis in the leading strand.
What's the difference between chromatin and chromatid?
How do the different DNA nucleotides pair during DNA replication?
What happens to mRNA that includes introns?
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How is bacterial DNA replication different from eukaryotic DNA replication?
If the upper strand of the DNA molecule below acts as a template to make mRNA, what is the nucleotide
Why is DNA polymerase a required part of PCR? a. It synthesizes proteins. b. It synthesizes new DNA. c. It separates RNA from DNA. d. It degrades the template DNA.
How are the three types of RNA different?
Determine the sequence of mRNA obtained from the transcription of the DNA strand. Coding strand: 5' - ATGGGTTGA -3' Template strand: 3' TACCCAACT -5'
Why is 3 nucleotides per codon better than 4 nucleotides per codon?
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Explain how mRNA contains information from a gene.
The strand shown below is the 1st strand of cDNA made from an mRNA sequence. 5' GCTACCGTACATG 3' Which of the following could have been the mRNA sequence? A. 5' GCTACCGTACATG 3' B. 5' GCTUCCGTUCATG 3' C. 5' CAUGUACGGUAGC 3' D. 5' GCUACCGUACAUG 3'
What are the benefits of mitochondrial DNA and ribosomal RNA?
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Use the polypeptide below to construct the mRNA strand as well as the original DNA code. Peptide: MET-LYS-AGR-PHE-GLY-PRO-STOP mRNA: Template:
The DNA replication enzyme that most closely resembles RNA polymerase is: a) DNA polymerase I. b) DNA polymerase III. c) primase. d) telomerase. e) helicase.
Why does adding a specific complementary nucleic acid sequence that can bind to mRNA transcribed from the superantigen gene prevent bacterial synthesis of the superantigen protein? Explain.
How are DNA and protein synthesis related?
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Transcription and Translation. Below is DNA coding strand. Use it to produce the template strand. Then, use the template strand to produce a strand of mRNA. Below that, using the dictionary of the genetic code, determine the polypeptide that the mRNA woul
What does a transcription factor do? a. Binds DNA and holds it together b. Binds RNA polymerase and makes proteins c. Binds to DNA and recruit mRNA-making machinery d. Binds to DNA and recruits othe
How are DNA and RNA involved in protein synthesis?
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The phrases or terms describe different fundamental processes of nucleic acids. Classify each phrase or term as relating to replication, transcription, or translation. 1. both DNA strands are duplicated 2. DNA polymerase 3. requires tRNA 4. described as s
How many types of mRNA are transcribed from the lac operon?
How does DNA polymerase distinguish between rNTP & dNTP?
How is transcription different in eukaryotic and prokaryotic cells?
How do codons and anticodons work during protein synthesis?
RNA polymerases differ from DNA polymerases in that \\ A. RNA polymerases do not require a template B. RNA polymerases use deoxyribonucleoside triophosphates as substrates C. DNA polymerases use ribonucleoside triphosphates as substrates D. RNA polymerase
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Describe the function of RNA in the transcription and translation of a gene that encodes a protein.
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What is the effect of a mutation that changes an mRNA strand from AUG CCC AAC UGA to AUG CCU AAC UGA ?
If an mRNA sequence is 5'AUG-GGC-ACU-CAU3', what would the anticodon sequence be a. 3'AUG-GGC-ACU-CAU5' b. 3'UAC-CCG-UGA-GUA5' c. 5'AUG-GGC-ACU-CAU3' d. 5'UAC-CCG-UGA-GUA3'

What are the differences and similarities between cDNA and mRNA? Why is cDNA used as a template...

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Genomics:-

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