The molecule below was spotted on a TLC plate and eluted with 1:1 ethyl acetate:methylene chloride. What would you expect the relative value of Rf to be after the elution? Explain your answer.
Thin Layer Chromatography:
In thin layer chromatography, compounds are separated and travel along the TLC plate due to their interaction with the mobile phase (solvent system). When the solvent system is non-polar, molecules interact more strongly with the TLC plate and therefore travel less distance along the TLC plate. With a very polar solvent, molecules would travel a greater distance as they interact more with the mobile phase.
Answer and Explanation: 1
Since this compound has a negative charge, it would not travel very far if at all on the TLC plate. It would be interacting too strongly with the...
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fromChapter 1 / Lesson 9
Understand what the retention factor in chromatography is by learning its definition. Learn how to calculate retention factor using the retention factor equation.